Screening using Mass Spectrometry

AliX has developed a unique and innovative platform using non-covalent mass spectrometry for compound screening and binding characterization. This screening technology has emerged as an alternative and efficient technique for delivering high-content information on compound-protein interactions:

• Ligand binding is evidenced through accurate mass measurement of intact complexes
• Binding stoichiometry is resolved from the difference of mass between free and bound protein
• Sequential competition experiments ensure compound binding specificity
• Collision-induced dissociation and determination of the Vc50 tell on the strength of the interaction in the gas phase
• Equilibrium affinity constant (KD) can be evaluated
• Ranking of molecule series is achieved through direct competition experiments